Journal: Discover Oncology

Article Title: Exosomal TACSTD2 promotes invasion, metastasis and glycolysis in ovarian cancer

doi: 10.1007/s12672-025-04041-6

Figure Lengend Snippet: Exosomal TACSTD2 promotes progression by mediating glycolysis in OC cells and exosomal TACSTD2 promotes progression through the ERBB2/PI3K/AKT/FOXO1 signaling pathway in OC cells. a , b The glycolysis inhibitor 2-DG reversed the promoting effect of of exosomal TACSTD2 on the migration and invasion of OC cells detected by Transwell assay. c Effect of exosomal TACSTD2 on ERBB2/PI3K/AKT/FOXO1 signaling pathways in OC cells detected by Western blot. d The PI3K inhibitor (LY294002) reversed the overexpression of the pathway proteins p-PI3K, p-AKT, and p-FOXO1 in OC cells detected by Western blot. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The immunoblots were probed with the following primary antibodies: TACSTD2 (1:1000; Bioworld, Minisuda, USA), GAPDH (1:10000; Bioworld, Minisuda, USA), CD9 (1:1000; Proteintech, Wuhan, China), CD63 (1:200; Proteintech, Wuhan, China), TSG101 (1:2000; Proteintech, Wuhan, China), ERBB2 (1:2000; Proteintech, Wuhan, China), PI3K (1:1000; Abmart, Shanghai, China), p-PI3K (1:500; Affinity, Cincinnati, USA), AKT (1:1000; Abmart, Shanghai, China), p-AKT (1:1000; Abmart, Cincinnati, USA), FOXO-1 (1:1000; Affinity, Cincinnati, USA), p-FOXO-1 (1:1000; Affinity, Cincinnati, USA), GLUT1 (1:1000; Affinity, Cincinnati, USA), HK2 (1:1000; Affinity, Cincinnati, USA), PKM2 (1:1000; Affinity, Cincinnati, USA), and LDHA (1:1000; Affinity, Cincinnati, USA).

Techniques: Migration, Transwell Assay, Protein-Protein interactions, Western Blot, Over Expression

Journal: OncoTargets and Therapy

Article Title: Exosome-Delivered EGFR Induced by Acidic Bile Salts Regulates Macrophage M2 Polarization to Promote Esophageal Adenocarcinoma Cell Proliferation

doi: 10.2147/OTT.S437560

Figure Lengend Snippet: Discription of Antibodies Used in the Study

Article Snippet: p-PI3K , MBS624519 , Rabbit monoclonal , Western blot , MyBiosource.

Techniques: Western Blot, Immunohistochemistry, Control

Journal: Molecular Medicine Reports

Article Title: Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells

doi: 10.3892/mmr.2023.13068

Figure Lengend Snippet: Primer sequences.

Article Snippet: Total proteins (30 μg) were separated by SDS-PAGE on 10% gels and were transferred to polyvinylidene fluoride membranes (cat. no. ISEQ00010; MilliporeSigma.), which were blocked with 5% skimmed milk at room temperature for 2 h. The membranes were then incubated with the following primary antibodies at 4°C overnight: Total (t)-IRS-1 (cat. no. ab52167; Abcam), rabbit antibody, 1:1,000; phosphorylated (p)-IRS-1 (cat. no. ab5599; Abcam), rabbit antibody, 1:1,000; t-AKT (cat. no. BS2987; Bioworld Technology, Inc.), rabbit antibody, 1:2,000; p-AKT (cat. no. BS4006; Bioworld Technology, Inc.), rabbit antibody, 1:2,000; t-PI3K (cat. no. 20583-1-AP; Proteintech Group, Inc.), rabbit antibody, 1:2,000; p-PI3K (cat. no. 11508; SAB Biotherapeutics, Inc.), rabbit antibody, 1:2,000; GLUT4 (cat. no. ab65267; Abcam), mouse antibody, 1:2,000; DDIT4 (cat. no. ab106356; Abcam), rabbit antibody, 1:2,000; t-mTOR (cat. no. ab32028; Abcam), rabbit antibody, 1:1,000; p-mTOR (cat. no. ab109268; Abcam), rabbit antibody, 1:1,000; t-p70S6K (cat. no. ab32529; Abcam), rabbit antibody, 1:1,000; p-p70S6K (cat. no. ab59208; Abcam), rabbit antibody, 1:1,000; β-actin (cat. no. 20536-1-AP; Proteintech Group, Inc.), mouse antibody, 1:5,000.

Techniques: Sequencing

Journal: Molecular Medicine Reports

Article Title: Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells

doi: 10.3892/mmr.2023.13068

Figure Lengend Snippet: PA induces lipid deposition and insulin resistance in C2C12 cells. (A) Glucose concentration in the culture medium was increased after PA treatment for 24 h. (B) Oil red O staining of C2C12 cells revealed lipid deposition 24 h after PA treatment; magnification, ×400, scale bar, 100 µm. (C) TG content was increased in C2C12 cells after PA treatment. (D) Effects of PA treatment on the mRNA expression levels of insulin pathway indicators (IRS-1, PI3K, AKT and GLUT4) in C2C12 cells. (E) Effects of PA treatment on the protein expression levels of insulin pathway indicators in C2C12 cells. (F) Semi-quantification of p-protein/t-protein ratios for insulin pathway indicators (IRS-1, PI3K, AKT) and protein expression levels of GLUT4 in C2C12 cells after PA treatment. All cells were treated with 100 nM insulin for 30 min before (A) detection of glucose contents and (E) western blotting. Data are presented as the mean ± SD (n=3). *P<0.05 vs. CON, # P<0.05 vs. PA1 (0.1 mM), & P<0.05 vs. PA2 (0.25 mM). CON, control; PA, palmitic acid; IRS-1, insulin receptor substrate-1; GLUT4, glucose transporter 4; p-, phosphorylated; t-t, total; TG, triglyceride.

Article Snippet: Total proteins (30 μg) were separated by SDS-PAGE on 10% gels and were transferred to polyvinylidene fluoride membranes (cat. no. ISEQ00010; MilliporeSigma.), which were blocked with 5% skimmed milk at room temperature for 2 h. The membranes were then incubated with the following primary antibodies at 4°C overnight: Total (t)-IRS-1 (cat. no. ab52167; Abcam), rabbit antibody, 1:1,000; phosphorylated (p)-IRS-1 (cat. no. ab5599; Abcam), rabbit antibody, 1:1,000; t-AKT (cat. no. BS2987; Bioworld Technology, Inc.), rabbit antibody, 1:2,000; p-AKT (cat. no. BS4006; Bioworld Technology, Inc.), rabbit antibody, 1:2,000; t-PI3K (cat. no. 20583-1-AP; Proteintech Group, Inc.), rabbit antibody, 1:2,000; p-PI3K (cat. no. 11508; SAB Biotherapeutics, Inc.), rabbit antibody, 1:2,000; GLUT4 (cat. no. ab65267; Abcam), mouse antibody, 1:2,000; DDIT4 (cat. no. ab106356; Abcam), rabbit antibody, 1:2,000; t-mTOR (cat. no. ab32028; Abcam), rabbit antibody, 1:1,000; p-mTOR (cat. no. ab109268; Abcam), rabbit antibody, 1:1,000; t-p70S6K (cat. no. ab32529; Abcam), rabbit antibody, 1:1,000; p-p70S6K (cat. no. ab59208; Abcam), rabbit antibody, 1:1,000; β-actin (cat. no. 20536-1-AP; Proteintech Group, Inc.), mouse antibody, 1:5,000.

Techniques: Concentration Assay, Staining, Expressing, Western Blot, Control

Journal: Molecular Medicine Reports

Article Title: Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR pathway in C2C12 cells

doi: 10.3892/mmr.2023.13068

Figure Lengend Snippet: Resveratrol ameliorates insulin resistance by improving insulin signaling via the DDIT4/mTOR/IRS-1/PI3K/AKT/GLUT4 pathway. IR, insulin resistance; PA, palmitic acid; DDIT4, DNA damage-inducible transcript 4; p70S6K, p70 ribosomal protein S6 kinase; IRS-1, insulin receptor substrate-1; GLUT4, glucose transporter 4.

Article Snippet: Total proteins (30 μg) were separated by SDS-PAGE on 10% gels and were transferred to polyvinylidene fluoride membranes (cat. no. ISEQ00010; MilliporeSigma.), which were blocked with 5% skimmed milk at room temperature for 2 h. The membranes were then incubated with the following primary antibodies at 4°C overnight: Total (t)-IRS-1 (cat. no. ab52167; Abcam), rabbit antibody, 1:1,000; phosphorylated (p)-IRS-1 (cat. no. ab5599; Abcam), rabbit antibody, 1:1,000; t-AKT (cat. no. BS2987; Bioworld Technology, Inc.), rabbit antibody, 1:2,000; p-AKT (cat. no. BS4006; Bioworld Technology, Inc.), rabbit antibody, 1:2,000; t-PI3K (cat. no. 20583-1-AP; Proteintech Group, Inc.), rabbit antibody, 1:2,000; p-PI3K (cat. no. 11508; SAB Biotherapeutics, Inc.), rabbit antibody, 1:2,000; GLUT4 (cat. no. ab65267; Abcam), mouse antibody, 1:2,000; DDIT4 (cat. no. ab106356; Abcam), rabbit antibody, 1:2,000; t-mTOR (cat. no. ab32028; Abcam), rabbit antibody, 1:1,000; p-mTOR (cat. no. ab109268; Abcam), rabbit antibody, 1:1,000; t-p70S6K (cat. no. ab32529; Abcam), rabbit antibody, 1:1,000; p-p70S6K (cat. no. ab59208; Abcam), rabbit antibody, 1:1,000; β-actin (cat. no. 20536-1-AP; Proteintech Group, Inc.), mouse antibody, 1:5,000.

Techniques: